Tissue morphology was studied using routine histologic, histochemical and immunohistochemical techniques. ![]() Rat lumbar intervertebral discs were maintained in organ culture in media supplemented with TGF-beta3 or TGF-beta1 for 1 week. Development of a useful organ culture model would enhance understanding of disc function in health and disease. To develop an in vitro organ culture of rat intervertebral disc and evaluate effects of TGF-beta3 on disc cell function.Īn in vitro model system is of considerable value in understanding the cell biology of the intervertebral disc. Role of TGF-beta isoforms on cell function was evaluated. ![]() ![]() Rat lumbar discs comprising nucleus pulposus, annulus fibrosus, and cartilaginous endplates were cultured for 1 week in a specialized media containing either TGF-beta1 or TGF-beta3.
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